Thermoactinomyces intermedius

J. J. Venit, L. J. Szarka, and R. N. Patel, Synthesis of allysine ethylene acetal using phenylalanine dehydrogenase from Thermoactinomyces intermedius, Enzyme Microb. Technol. 2000b, 26, 348-358. 

PheDH from Thermoactinomyces intermedius ATCC 33 205 was utilized recently to synthesize allysine ethylene acetal [(S)-2-amino-5-(l,3-dioxolan-2-yl)-pentanoic acid (2)] from the corresponding keto acid with regeneration of NAD+ cofactor by FDH/ formate[58 (Fig. 15.3-4) the specific activity towards the keto acid was 16% compared to the standard substrate phenylpyruvate. 

Thermoactinomyces intermedius and endogenous formate dehydrogenase. The reductive amination process was further scaled up using a preparation of the two enzymes expressed in single recombinant E. coli. The amino acid 7 can be directly protected as its boc derivative without isolation to afford intermediate 8. Yields before isolation were close to 98% with 100% EE. 

Chen, J. Harris, K. Natalie, K. Ramig, S. Swaminathan, V. Rosso, S. Pack, B. Lotz, P. Bemot, A. Rusowicz, D. Lust, K. Tse, J. Venit, L. Szarka, R.N. Patel, Synthesis of allylsine ethylene acetal using phenylalanine dehydrogenase from Thermoactinomyces intermedius, Enzyme Microb. Technol. 26 (2000) 348-358. 

PheDH NAD Bacteria Sporosarcina ureae. Bacillus sphaericus, Rhodococcus marinas, Thermoactinomyces intermedius)... 

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