Thermo-optical absorbance detection

Protein Sequencing Using Microreactors and Capillary Electrophoresis with Thermo-optical Absorbance Detection... [Pg.3]

A miniaturized protein and peptide microsequencer consisting of either a fused silica capillary reactor or a microreactor made of Teflon is described. The performance of the miniaturized sequencer was evaluated by sequencing 33 and 27 picomoles of myoglobin that were covalently attached to Sequelon-DITC. The products generated by the sequencer were analyzed using capillary electrophoresis with thermo-optical absorbance detection. This CE system provides reproducible migration time (< 0.4% of RSD) and detection limits of less than 4 fmol. [Pg.3]

Yu and Dovichi [10] used capillary zone electrophoresis with thermo-optical absorbance detection to determine sub pg Lconcentrations of 18 amino acids. [Pg.234]

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