Theories of retention

R. E. Boehm and D. E. Martiie, A unified theory of retention and selectivity in liquid chromatography. 1. Eiquid-solid (adsorption) clrromatography , J. Phys. Chem. 84 3620-3630(1980). 

Colin, H. and Krstulovic, A. M., The theory of retention in reversed-phase high-performance liquid chromatography, in Liquid Chromatography in Pharmaceutical Development, Wainer, I. W., Ed., Aster Publishing, 1985, 171. 

A discussion on the theories of retention mechanisms and the type of forces generating retention and selectivity is not intended in this chapter. The interactions between solute, eluent components, and stationary phases with inhomogeneous surfaces are based on molecular recognition, where our understanding is still very limited. Therefore, an empirical description of retention and selectivity is preferred. 

DE Martire, RE Boehm. Unified theory of retention and selectivity in liquid chromatography. 2. Re-versed-phase liquid chromatography with chemically bonded phases. J Phys Chem 87 1045-1062, 1983. 

To illustrate the approach to the theory of retention and band broadening, we examine some aspects of programmed temperature gas chromatography. ... 

The development of a theory of retention and band broadening for macro-molecular HPLC is intended primarily for improving such separations. We need to relate various separation goals to (1) experimental conditions, (2) the choice of column type and HPLC method (RPLC, SEC, etc.), and (3) the nature of the sample. The present model allows os to do this 1 prediction, rathor than by experiment Our main requirement is usuaOy to achieve adequate separation, or some minimum resolution R, betw adjacent bands of interest. In this section we will show that the separation of macro-molecular samples by either isocratic or gradient elution is understandable and controllable, using the same concepts that we use for optimizing the isocratic separation of small molecules. 

In general, the theory of gas-solid chromatography consists oif two main parts, viz. theory of retention and theory of zone dispersion in gas-solid chromatography [14, 15]. 

The theories of retention and efficiency (HETP) reflect the main features of adsorption capillary columns. 

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