The surface chemistry of silica SEC packings

Polar synthetic polymers or biopolymers are chromatographed on SEC silica columns employing aqueous salt or buffer solutions 

With increasing eluent pH the equilibrium of eq.(23) is shifted to the right, which simultaneously leads to a higher negative charge density of the silica, while the acidity of the remaining 

Native silicas are employed In SEC of polar synthetic poly- 

Bonded phases are usually characterized by the ligand density of the functional groups derived from elemental analysis, and the specific surface area of the notive silica, assuming a defined 

TABLE II Siiones employed in the synthesis of hydrophilic bonded silicas 

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