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The Antilectin Activity of AFGP

The active AFGP 1-5 were first reported by Chuba and co-workers (1973) to inhibit hemagglutination by a lectin prepared from the seeds of the Osage orange (Maclura pomifera). Although the structural requirements for the antilectin activity closely parallel those for antifreeze activity, these two activities appear to be unrelated, with the probable exception that they both require considerable integrity of the carbohydrate side chain and polymeric size. [Pg.219]

Active and inactive AFGP tested at 100 /ig/ml starting concentration also failed to inhibit numerous other lectins as well as immune animal sera possessing various hemagglutinating specificities. [Pg.219]

Agglutinating reagent Erythrocytes tested 1 8 Titer agglutinating reagent mixed with equal volume of  [Pg.220]

The quantities of antifreeze glycoproteins required for the inhibition of the lectin and depression of the freezing temperature are 0.1 pg and 1.0 mg, respectively that is, the lectin assay is 104 times more sensitive. The lectin therefore provides a micro method for determining the amounts of the glycoprotein in tissues and fluids of polar fishes and for studying its biosynthesis and metabolism. [Pg.221]


Although borate was also found to inhibit the antilectin activity of AFGP, the results were more difficult to interpret because borate inhibited the activity of the lectin itself in the hemagglutination assay. [Pg.242]


See other pages where The Antilectin Activity of AFGP is mentioned: [Pg.191]    [Pg.219]   


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