Thaumatin, crystal

Fig. 11.11 X-ray diffraction pattern from a single thaumatin crystal grown and harvested from a 5 nL chip micro-well. The inset shows a clean reflection at 1.35 A resolution.

C. E., Thaumatin crystallization aboard the International Space Station using liquid-liquid diffusion in the Enhanced Gaseous Nitrogen Dewar (EGN). Acta Crys-tallogr.. Sect D, Biol. Crystallogr. 2002, 58 (5), 751-760. 

Malkin, A.J. Kuznetsov, Y.G. Glantz, W. McPherson, A. Atomic force microscopy studies of surface morphology and growth kinetics in thaumatin crystallization. J. Phys. Chem. 1996, 100 (28), 11,736-11,743. 

FIGURE 6.22 Diffraction images frequently reveal problems with particular crystals that are sometimes blatant but occasionally subtle. These include disorder, multiple crystals, or twinned crystals. In (a), the pattern initially appears very ordered and proper, but close inspection of the row of reflections indicated provides evidence that this monoclinic thaumatin crystal is in fact twinned. In (b), the reflections from a Bence-Jones protein crystal fall not on a single reciprocal lattice but multiple, interwoven lattices indicative of twinned or multiple crystals. In (c), a tetragonal crystal of Bence-Jones protein is seriously disordered as evidenced by the smeared, highly mosaic reflections and high background scatter. 

FIGURE 3.1 Crystals of a variety of macromolecules. In (a), clusters of needle crystals of bacterial a amylase in (b), crystals of a protease from pineapple, in (c), a crystal of satellite tobacco mosaic virus and in (d), a crystal of the sweet protein thaumatin. 

Spectrometric investigation O. Korver et at.. Eur. J. Biochem. 35, 554 (1973). Studies on the primary structure of thaumatin 1 R. B. Iyengar et al. Ibid. 96, 193 (1979). Thaumatins and 11 consist of almost identical sequences of 207 amino acids. Thaumatin I crystallizes in two different forms H. van der Wei et al. FEBS Letters 56, 316 (1975). The crystal structure shows two distinct regions with the amino acids either in sheets or in complex loops. Crystal... 

Droplet-based microfluidics have also been employed for a number of unique chemical reactions including precipitation reactions [76,77] synthesis of cobalt [78] and titania [79], titration of formic acid [80] and anticoagulants [38], and crystal growth [81]. Lau et al. [82] examined 40 different conditions within a single device to crystallize catalase, glucose isomerase, thaumatin, and ferritin. 

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