TFIIIC TFIIIB interactions

Eukaryotic cells contain multiple copies of the 5S rRNA gene. Unlike other eukaryotic rRNA genes, the 5S rRNA genes are transcribed by RNA Pol III. Two control elements, an A box and a C box, lie downstream of the transcriptional start site. The C box binds TFIIIA which then recruits TFIIIC. TFIIIB now binds and interacts with RNA Pol III to form the transcription initiation complex. Transcription produces a mature 5S rRNA that requires no processing. 

VI. Ordered Binding of TFIIIB Subunits to TFIIIC-DNA and Dynamic Interactions with Tfc4... 

Bartholomew, B., Kassavetis, G. A., and Geiduschek, E. P. (1991). Two components of Saccharomyces cerevisiae transcription factor IIIB (TFIIIB) are stereospecifically located upstream of a tRNA gene and interact with the second-largest subunit of TFIIIC. Mol. Cell. Biol. 11, 5181-5189. 

Chaussivert, N., Conesa, C., Shaaban, S., and Sentenac, A. (1995). Complex interactions between yeast TFIIIB and TFIIIC. J. Biol. Chem. 270, 15353-15358. 

Hsieh, Y. J., Wang, Z., Kovelman, R., and Roeder, R. G. (1999b). Cloning and characterization of two evolutionarily conserved subunits (TFIIIC102 and TFIIIC63) of human TFIIIC and their involvement in functional interactions with TFIIIB and RNA polymerase III. Mol. Cell. Biol. 19, 4944-4952. 

---