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Tetramethylrhodamine isothiocyanate derivative

Tetramethylrhodamine-5-(and 6)-isothiocyanate (TRITC) is one of the most popular fluorescent probes available. The isothiocyanate derivative of tetramethylrhodamine is synthesized by... [Pg.416]

The red-emitting rhodamine derivatives are constructed around the same basic xanthene framework as is fluorescein (2). Tetramethylrhodamine isothiocyanate (TRITC) has been widely employed for immunofluorescence. Additional derivatives of rhodamine available for conjugation to antibodies include lissamine rhodamine sulfonyl chloride (RB-200-SC), rhodamine B isothiocyanate (RBITC), rhodamine X isothiocyanate (XRITC), and Texas... [Pg.101]

The following method is used to fluorescentiy label amine groups (present for example in lysines) with the rhodamine derivative tetramethylrhodamine-5-isothiocyanate (TRUC excitation = 546 nm, emission 579 nm). Following this protocol a labelling efficiency of two to four moles of dye per mole of antibody is usually achieved. [Pg.357]

See other pages where Tetramethylrhodamine isothiocyanate derivative is mentioned: [Pg.207]    [Pg.207]    [Pg.207]    [Pg.207]    [Pg.190]    [Pg.536]    [Pg.129]    [Pg.1336]    [Pg.309]    [Pg.1386]    [Pg.743]   
See also in sourсe #XX -- [ Pg.416 ]



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Tetramethylrhodamine

Tetramethylrhodamine-5 -isothiocyanate,

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