Tetrabromophenol Blue

Other Names Phenol, 4,4 -(4,5,6,7-tetrabromo-3H-2,l-benzoxathiol-3-yIidene)-feis[2,6-dibromo-, 5,5-dioxide 3H-2,l-Benzoxathiole, phenol derivative NSC 11236 Tetrabromphenol bine CA Index Name Phenol, 4,4 -(4,5,6,7-tetrabromo-l,l-dioxido-3H-2,l-benzoxathiol-3-ylidene)i w [2,6-dibromo- 

CAS Registry Number 4430-25-5 Merck Index Number Not listed Chemical Structure 

Chemical/Dye Class Sulfonephthalein Molecular Formula Ci9H6Brg05S Molecular Weight 985.54 pH Range 3.0-4.6 

Physical Form Off-white to pale lavender powder Solubility Soluble in water, ethanol, methanol, acetone, acetic acid UV-Visible ( ) 610 nm, 388 nm Melting Point 204°C (decompose) 

Boiling Point (Calcd.) 728.8 60.0°C Pressure 760 Torr Synthesis Synthetic methods  

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Tetrabromophenol blue, tetrabromophenol-tetrabromosulfonphthalein (indicator) dissolve 0.1 g in 5 mL 0.02/7 NaOI I and dilute with water to 250 mL pH range yellow 3.0-4.6 blue. 

Fig. 18 Chemical structure of tetrabromophenol blue and the color change after the reaction with human serum albumin (HSA)...

To make use of their merits and to erase their drawbacks, we carried out the multicolor visual colorimetry over a large concentration range of protein under the optimum mixing ratio of tetrabromophenol blue and eosin B using L a b color coordinates based on DCA. 

Fig. 20 Color variation of the mixed dyes (tetrabromophenol blue and eosin B) on the a b color coordinate for the response to the various concentrations of HSA (a) and their color sample (b) by simulation...

Albustix contains the indicator, tetrabromophenol blue, in a citrate buffer at pH 3. At this pH, the indicator has a yellow color. In the presence of protein, the color changes to green. The higher the protein concentration, the greener the indicator will be. Therefore, the color produced by the Albustix can be used to estimate the concentration of protein in urine. 

Yoshida, T., Yoshimura,)., Matsui, M Sugiura, T., and Minoura, H. (1999) Self-assembly of ZnO/ tetrabromophenol blue mixed thin film by one-step electrodeposition and its sensitized photoelectrochemical performance. Trans. Mater. Res. Soc. Jpn., 24,497-500. 

Tetrabromoethane s-Tetrabromoethane. See Tetrabromoethane 2,4,5,7-Tetrabromo-3,6-fluorandiol. See D C Red No. 21 Solvent red 43 Tetrabromofluorescein 2, 4, 5, 7 -Tetrabromofluorescein. See Solvent red 43 2,2, 6,6 -Tetrabromo-4,4 -isopropylidenediphenol. See Tetrabromobisphenol A Tetrabromomethane. See Carbon tetrabromide Tetrabromophenol blue Tetrabromophenol sulfophthalein. See Bromophenol blue Tetrabromophthalate diol CAS 20566-35-2 EINECS/ELINCS 243-885-0 Empirical Ci5Hi6Br407 Properties Amber vise, liq. sol. in acetone, toluene insol. in water, alcohol m.w. 627.90 dens. 1.80 kg/l... 

Lin, G. H. Y Brusick, D. J. Mutagenicity studies on two triphenylmethane dyes, bromophenol blue and tetrabromophenol blue. J. Appl. Toxicol. 1992,12, 267-274. 

A dipstick test manufactured by Ames for the estimation of protein in urine. It is based on the change of colour of tetrabromophenol blue from yellow to green caused by proteins, especially albumin. Alkaline urines may give false positive results. 

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