Targeting peptide prediction

Fig. 1.3 Prediction of the most appropriate subcellular targeting strategies by agroinfiltration. The levels of an industrial enzyme (IE) are shown in agroinfiltrated and transgenic alfalfa leaves using different subcellular targeting peptides. Equal amounts of total soluble leaf proteins were separated by SDS-PAGE and blotted onto a PVDF membrane. Polyclonal anti-IE IgGs were used for detection.

It has been suggested that an FeS flavoprotein with presumed electron transporting function may be involved in the reduction of the flavodiiron protein in Methanosarcina acetivorans (Saraiva et al. 2004). Since putative FeS flavoproteins with predicted hydrogenosomal target peptides have also... 

Emanuelsson, O., G. von Heijne, and G. Schneider. 2001. Analysis and prediction of mitochondrial targeting peptides. Methods Cell Biol 65 175-87. 

Target (htq) //www.cbs.dtu.dk/servicesA argetP/) assigns the subcellular location of proteins based on the predictions of the N-terminal chloroplast transit peptide, mitochondrial targeting peptide or secretory signal peptide. 

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