Multiple reaction monitoring tandem mass spectrometry

Selected Reaction Monitoring (SRM) Data acquired from specific product ions corresponding to m/z selected precursor ions recorded via two or more stages of mass spectrometry. Selected reaction monitoring can be preformed as tandem mass spectrometry in time or tandem mass spectrometry in space. The term multiple reaction monitoring is deprecated [1],... 

Figure 5.64 LC-UV and LC-MS-MS (multiple-reaction monitoring (MRM)) traces from the analysis of a synthetic mixture of four native and five oxidized deoxynucleosides (for nomenclature, see text). Reprinted by permission of Elsevier Science from Comparison of negative- and positive-ion electrospray tandem mass spectrometry for the liquid chromalography-landem mass speclrometry analysis of oxidized deoxynucleosides , by Hua, Y., Wainhaus, S. B., Yang, Y., Shen, L., Xiong, Y., Xu, X., Zhang, F., Bolton, J. L. and van Breemen, R. B., Journal of the American Society for Mass Spectrometry, Vol. 12, pp. 80-87, Copyrighl 2000 by Ihe American Society for Mass Spectrometry.

Gergov M, Ojanpera I, Vuori E. 2003. Simultaneous screening for 238 drugs in blood by liquid chromatography-ionspray tandem mass spectrometry with multiple-reaction monitoring. J Chromatogr B 795 41. 

Table 2.1.3 Multiple reaction monitoring of amino acids for their tandem mass spectrometry quantitation. In daily practise not all mentioned amino acids are measured in one run, but a set of ten dedicated evaluation programs has been developed, covering groups of amino acids associated with groups of disorders. Amino acids presented in italics indicate stable-isotope-labeled internal standards ... 

Table 5.4.12 The optimum HPLC-tandem mass spectrometry (MS/MSj parameters for each BA (adapted from [31]). CAD Collision-activated dissociation, MRM multiple reaction monitoring...

Gschwend MH, Arnold P, Ring J, Martin W (2006) Selective and sensitive determination of amisulpride in human plasma by liquid chromatography-tandem mass spectrometry with positive electrospray ionisation and multiple reaction monitoring. J Chromatogr B Analyt Technol Biomed Life Sci 831(1-2) 132-139. doi S1570-0232(05)00890-l [pii] 10.1016/j.jchromb.2005.11.042... 

Fig. 5 Statistical evaluation of LC-MS-based methods for tropane alkaloids referred in this chapter. (a) Relative frequency of ionization methods. +APCI positive atmospheric pressure chemical ionization, +ESI positive electrospray ionization, FAB fast atom bombardment, +TSP positive thermospray, (b) Relative frequency of scan modes used. MS full scan MS, MS/MS tandem mass spectrometry (product ion scan), MRM multiple reaction monitoring, SIM selected ion monitoring, (c) Relative frequency of mass analysers used. EBQtQ2 double focusing sector field mass spectrometer, IT ion trap, QqQ triple quadrupole, SQ single quadrupole. Considered publications were found by PubMed data-based search and references cited in these articles...

Multiple Reactions Monitoring, also Metastable Reaction Monitoring Mass Spectrometry Tandem Mass Spectrometry... 

High-performance liquid chromatography-tandem mass spectrometry method for the determination of gemifloxacin in human plasma was based on the protein precipitation of plasma samples with acetonitrile containing [ C Ha] gemifloxacin as an internal standard. The supernatant was injected onto a PLRP-S column without any further clean-up. The mass spectrometer was operated in positive ion mode, and the ions were detected in multiple reaction-monitoring (MRM) mode. The assay requires 50 gl of plasma and is precise and accurate within the range 10-5000 ng/ml [15]. 

A liquid chromatography-tandem mass spectrometry (LC-MS-MS) method was developed to detect tumor-promoting diterpene esters of the tigliane and ingenane types within plant extracts. Fractionation on a Cig HPLC column was followed by MS-MS-multiple reaction monitoring (MRM). 

An alternative method of overcoming interferences in organic analysis is to utilise tandem mass spectrometry (MS-MS). This technique is now popular, particularly in the LC-MS field, and offers the means to overcome interferences without recourse to the expensive and increasingly rare high resolution instrumentation. By utilising MS-MS in the multiple reaction monitoring (MRM) mode IDMS can readily be carried out, ... 

Liquid chromatography, coupled with mass spectrometry and tandem mass spectrometry (LC-MS/MS), is a universal technique for determining toxins in food. The first multiple reaction monitoring (MRM) method for polyether marine toxins was used for the determination of DSP toxins and this technique has been successfully applied to the simultaneous determination of various groups of polyether toxins in shellfish (Draisci et al., 1998, 1999). MRM involves the selection of a precursor ion and its product ion from CID fragmentations and it is highly selective. For azaspiracids, the first validated LC-MS/MS method was developed for the determination of AZAl... 

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