T-Sodium Hydroxide

Only theophylline yields an intensely fluorescent derivative under long-wavelength UV light when treated with chloramine T — sodium hydroxide reagent. The purine derivatives caffeine and theobromine investigated at the same time fluoresce very weakly or not at all. 

Digitalis glycosides that react well with various chloramine T - trichloroacetic acid or mineral acid reagents are not excited to fluorescence after treatment with chloramine T — sodium hydroxide. 

The phenols pyrocatechol, resorcinol and hydroquinone can be detected with all chloramine T reagents. The detection sensitivity is about the same with chloramine T - sodium hydroxide and chloramine T - trichloroacetic acid. In all cases the detection limits are ca. 75 ng substance per chromatogram zone after the plate has been subsequently dipped in a paraffin oil solution. Somewhat less favorable detection limits of 150 to 200 ng substance per chromatogram zone are obtained after treatment with chloramine T - hydrochloric acid and chloramine T - sulfuric acid. 

Imldes. Imides are generally water-soluble, consequently Hiey are much more readily hydrolysed in an alkaline medium, e.g., by refluxing with 10 per c t. sodium hydroxide solution ... 

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