Synthetic promoter libraries design

In order for fine-tuning of gene expression to be truly synthetic one should be able to design promoters with a desired strength in silico and then use these to obtain a certain phenotype (De Mey et aL 2007 Ellis et aL 2009). To this end Jensen et al. created a model that could predict whether a certain promoter in a library was weak or strong and identify critical sites in the promoters that led to either weak or strong activity. This qualitative model was a first step to link promoter sequence to promoter strength (Jensen et aL 2006). 

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