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Promoter strength

Nacken, V., Achstetter, T. and Degryse, E. (1996) Probing the hmits of expression levels by varying promoter strength and plasmid copy number in Saccharomyces cerevisiae. Gene, 175, 253-260. [Pg.282]

Chou W-Y, Stewart MJ, Carr LG, Zheng D, Stewart TR, Williams A, Pinaire J, Crabb DW. An A/G polymorphism in the promoter of mitochondrial aldehyde dehydrogenase (ALDH2) effects of the sequence variant on transcription factor binding and promoter strength. Alcohol Clin Exp Res 1999 26 963-968. [Pg.438]

Comparison of cellular promoters to viral promoters shows that the cellular promoters are weaker than the viral promoters, as reflected in the level of gene expression. However, addition of an enhancer element can increase promoter strength as noted above with the combination of the CMV enhancer and the cellular chick beta-actin promoter. [Pg.414]

In E. coli precise spacing between the conserved -35 and -10 (Pribnow) promoter elements has been found to be a critical determinant of promoter strength. What does this suggest about the interaction between RNA polymerase and these conserved sequences in the DNA ... [Pg.1667]

Each of these factors is again influenced by several parameters. The total amount of a protein expressed in an organism or tissue is determined by the rate of protein synthesis vs. degradation. In general, protein synthesis depends on the amount of mRNA produced (promoter-strength and temporal/spatial expression pattern) and its usability for the protein synthesis machinery. The... [Pg.61]

Influence of Promoter Strength in Lnciferase Gene Expression (Table U)... [Pg.255]

Blazeck, J. et al. (2012) Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters. Biotechnol Bioeng., 109 (11), 2884-2895. [Pg.782]


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See also in sourсe #XX -- [ Pg.499 ]




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