Synthetic chemicals consequences from

The atmosphere is the recipient of many of the products of our technological society. These effluents include products of combustion of fossil fuels and the development of new synthetic chemicals. Historically these emissions can lead to unforeseen consequences in the atmosphere. Classical examples include the realization in the 1950s that motor vehicle emissions could lead to urban smog and the realization in the 1970s that emissions of chlorofluorocarbons from aerosol spray cans and refrigerators could cause the depletion of stratospheric ozone. 

Soon after the hypothetical structure was published, coelenterazine was isolated as an actual substance from the liver of the luminous squid Watasenia scintillans, and it was chemically synthesized (Inoue et al., 1975). The availability of synthetic coelenterazine led to the important discovery that the treatment of the luminescence product of aequorin with coelenterazine results in the regeneration of active aequorin (Shimomura and Johnson, 1975c), which consequently confirmed the presence of a coelenterazine moiety in the aequorin molecule. During the same period, it became increasingly evident that coelenterazine is involved as a luciferin in various bioluminescent organisms, such as the sea cactus Cavernularia, the sea pen Ptilosarcus, and the sea pansy Renilla (Shimomura and Johnson, 1975b). 

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