Synthesis via Grafting-to Approach

Gianneschi and coworkers [29] have utilized a similar approach in functionalizing ROMP copolymers with hydrophilic peptides. This work involves a block copolymer synthesis incorporating a phenyl NBE moiety as the hydrophobic block and an NHS moiety as the chemically addressable block. Subsequent conjugation of peptides via their N-termini affords amphiphilic block copolymers in which the hydrophilic shell is defined by a short peptide sequence programmed to be an enzyme substrate. For graft-to functionalization, A/ ,A/ -diisopropylethylamine was added to the activated ester diblock copolymer in DMF, followed by the addition of peptide (with respect to NHS units). 

Sleiman and coworkers prepared DNA polymer conjugates by conjugation to two different types of activated ester-labelled ROMP polymer backbones 

Growing DNA with bases and phosphate groups attached 

Synthesis (i) a hydrophobic polymer, terminally modified with a carboxylic acid moiety, was mixed with a coupling agent and reacted with a 50-amino-modified oligonucleotide on solid support (controlled pore glass, CPG) (ii) deprotection and cleavage of the resulting DNA polymer conjugate from solid support (iii) dialysis of cleaved DPA into deionized water to form a mixture 

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