Synthesis of Poly deoxyribonucleotides

Homopoly(deoxyribonucleotides) up to the decamers may be rapidly obtained by polymerization of nucleotides (IV-protected, where necessary) and resolution of the resulting homologs. The DCC-cata- 

Primarily in order to eliminate chain scission during block condensation-reactions, leading to oligomers having incorrect sequences,229 

the methods depend upon preparation of the fully protected 2 -deoxyribonucleoside 3 -[(2-cyanoethyl) 8-quinolinyl phosphate]. The 8-quinolinyl group is conveniently removed by treatment with cupric chloride in a mixture of dimethyl sulfoxide and water. 

Use of DNA Ligase for the Synthesis of Double-stranded Poly(deoxyribonucleotides) 

The discovery of an enzyme that catalyzes the repair of defects in one strand of double-stranded DNA34,262,263 led to the now well-established technique for joining two adjacent segments when both are hydrogen-bonded to a third, splint oligomer (see formula 5). 

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