Synechococcus vulcanus

Bialek-Bylka GE, Fujii R, Chen C-H, Oh-oka H, Kamiesu A, Satoh K, Koike H and Koyama Y (1998b) 15-Cis-carotenoids found in the reaction center of a green sulfur bacterium Chlorobium tepidum and in the Photosystem I reaction center of a cyanobacterium Synechococcus vulcanus. Photosynth Res 58 1-8... 

Shen ]-R, Ikeuchi M, Inoue Y. Stoichiometric association of extrinsic cytochrome C550 and 12 kDa protein with a highly purified oxygen-evolving photosystem II core complex fi om Synechococcus vulcanus. FEBS Lett 1992 301 145-149. 

Koike H, Inoue Y. Properties of a peripheral 34-kDa protein in Synechococcus vulcanus Photosystem II particles. Its exchangeability with spinach 33-kDa protein in reconstitution of oxygen evolution. Biochim Biophys Acta 1985 807 64-73. 

Here we identified all the low-molecular-mass components from various types of PSII complexes by N-terminal sequencing and compared them between a thermophilic cyanobacterium, Synechococcus vulcanus. and higher plants. 

The Synechocystis 6803 psbH gene Is shorter than its chloroplast counterpart (Fig. 5). Uur data agrees with the reported 6.5 kD sizing and N-terminal sequencing of the Synechococcus vulcanus psbH polypeptide (9). The amino terminus appears truncated and lacks tTte TRf-2 phosphorylation site. It is doubtful that this polypeptide is phosphorylated in Synechocystis 6803. In addition psbH is not situated 3 to psbB in Synechocystis 6803 as is the conserved case in chloroplast genomes. 

The K protein has been shown to be present in PSll preparations but not in purified oxygen-evolvinq spinach PSll core preparations (5). However it has been detected in oxygen-evolving preparations from the cyanobacterium Synechococcus vulcanus (3). The mature form of the K protein is shorter than would be predicted from the gene, which suggests processing. Sequence comparisons between liverwort and tobacco indicate that both proteins are well conserved. 

Besides that, Co EMS was earlier applied for monitoring the state of cobalt(ll) in roots of water hyacinth Eichhomia crassipes [28], as well as in cells of a cyanobacterium (the blue-green alga Synechococcus vulcanus) [29] and Gram-negative bacteria (Escherichia coli [30] and, more recendy. Azospirillum brasilense in the freeze-dried state [27,32] or in frozen aqueous suspensions (FAS) [31,32] after their contact with Co" in solution). 

A similar EMS study was performed on Co in thermophilic cyanobacteria (blue-green alga, Synechococcus vulcanus) i ncubated with carrier-free salt for 4 months at 55 °C [29]. The cell suspension v/as centrifuged, and live cells were... 

The same analysis described above was applied to PS I RCs of Synechococcus Sc.) vulcanus (Bialek-Bylka et al., 1998b). Each of the Psa A and Psa B subunits is believed to contain 5-7 or 6-8 Carotene molecules (Thornberetal., 1991 Golbeck, 1992). Fig. 16d shows an HPLC elution profile for the acetone extract from the RC of Sc. vulcanus. The wavelengths of the vibrational structures of the A ... 

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