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Synchrotron Infrared Microscopes

Infrared microscopes are commercially available from a number of companies worldwide, and operate much like conventional visible light microscopes. The IR radiation follows the same path as the sample iUumination Ught, so that IR micro-spectroscopy can be performed on the sample at the center of the viewing field. Because of their design, they are also equipped with a number of convenient methods for enhanced sample visualization. These include polarized light (visible and IR), fluorescence illumination and differential interference contrast (DIG), all of which are well known and frequently used to identify biological sample histology. [Pg.457]

FT-IRM and FT-IRI instruments are designed with two paths from the sample to the detector, namely transmission and reflection  [Pg.457]


Figure 3. Broadband spectrum of a conventional 2000 Globar IR source (short dashed line), and the spectrum of the NSLS synchrotron source (solid line) limited by an experimental throughput of 4.4><1 O 4 mm2sr. This is the etendue for a 1 pm by 1 pm sample measured with an infrared microscope. The measured, background limited Noise Equivalent Power (NEP) of a Mercury Cadmium Telluride (MCT) (long dashed line) detector is shown. This detector is operated at liquid nitrogen temperatures. Figure 3. Broadband spectrum of a conventional 2000 Globar IR source (short dashed line), and the spectrum of the NSLS synchrotron source (solid line) limited by an experimental throughput of 4.4><1 O 4 mm2sr. This is the etendue for a 1 pm by 1 pm sample measured with an infrared microscope. The measured, background limited Noise Equivalent Power (NEP) of a Mercury Cadmium Telluride (MCT) (long dashed line) detector is shown. This detector is operated at liquid nitrogen temperatures.
Synchrotron Infrared spectroscopy has witnessed several important applications in Materials Science over the recent years. This chapter is aimed at highlighting the most recent studies that could inspire new studies from readers. Soft matter (in particular polymer science), catalysis and microscopic ellipsometry have achieved important steps forward in their applications recently, while well-established studies in semiconductors and high pressure studies have generated important results and findings. The field is evolving quickly towards new directions, mainly in the production of intense THz beams that are opening new research directions, in time resolved studies, in fast imaging and in near field infrared microscopy. The recent advances are reported in this chapter. [Pg.141]

Infrared microspectroscopy was used to examine numerous plant and animal tissues long before the union of the IR microscope and the synchrotron source [30]. For complex samples such as human tissues, an IR spectrum can provide a direct indication of sample biochemistry. [Pg.461]

The optical requirements for an IR microscope include (i) exact positioning of the sample (ii) spatial isolation of the sample from a larger matrix in the IR beam and (Hi) capability to function in both the visible and the infrared spectral regions. For infrared microspectrometry, a thermal emission source is generally used. Fourier transform spectrometers use interferometers as an effective means to resolve photon energies. Mercury cadmium telluride (MCT) detectors have the sensitivity and speed needed for FTIR spectrometers. The use of synchrotron radiation dramatically improves infrared microspectroscopy and has the power to analyse and map samples at high resolution. SR sources have transformed the IR microspectrometer into a true IR microprobe, providing IR spectra at the diffraction limit. Optics and performance of a /uF llR interfaced with SR were described [423]. Some 15 synchrotron beam lines are equipped with IR microscopes. [Pg.522]

Figure 2.8 Infrared signal intensity recorded using a confocal IR microscope with a single-point detector through various apertures using a synchrotron or globar source. Copyright 2005, with permission from Elsevier. Figure 2.8 Infrared signal intensity recorded using a confocal IR microscope with a single-point detector through various apertures using a synchrotron or globar source. Copyright 2005, with permission from Elsevier.
Figure 2.9 Plot demonstrating the small spot size that can be achieved using synchrotron-sourced mid-infrared radiation. The plot represents the integrated signal intensity from 2000-9000 cm through a 10 pm pinhole scanned on a microscope stage in an FT-IR spectrometer. Reproduced from reference [9] by kind permission of the Advanced Light Source (ALS), Berkeley Laboratory. Figure 2.9 Plot demonstrating the small spot size that can be achieved using synchrotron-sourced mid-infrared radiation. The plot represents the integrated signal intensity from 2000-9000 cm through a 10 pm pinhole scanned on a microscope stage in an FT-IR spectrometer. Reproduced from reference [9] by kind permission of the Advanced Light Source (ALS), Berkeley Laboratory.
Infrared signal measured through various aperture sizes using a synchrotron source versus a Globar source. Data collected with a confocal IR microscope and a single-point detector. (Reprinted from ref. 72.)... [Pg.91]

In infrared and Raman microscopes the sample is moved by very small increments along a plane perpendicular to the direction of illumination and the process is repeated until vibrational spectra for all sections of the sample are obtained. The size of a sample that can be studied by vibrational microscopy depends on a number of factors, such as the area of illumination, the power of the radiation delivered to the illuminated area, and the wavelength of the incident radiation. Up until the diffraction limit is reached, the smaller the area that is illuminated, the smaller the area from which a spectrum can be obtained. High radiant power is required to increase the rate of arrival of photons at the detector from small illuminated areas. For this reason, lasers and synchrotron radiation are the preferred radiation sources. Use of the best equipment makes it possible to examine areas as smcJl is 9 pm by vibrational microscopy. [Pg.484]


See other pages where Synchrotron Infrared Microscopes is mentioned: [Pg.457]    [Pg.153]    [Pg.457]    [Pg.153]    [Pg.458]    [Pg.445]    [Pg.2271]    [Pg.396]    [Pg.180]    [Pg.161]    [Pg.4]    [Pg.388]    [Pg.56]    [Pg.179]    [Pg.15]    [Pg.455]    [Pg.191]    [Pg.88]    [Pg.1723]    [Pg.385]    [Pg.98]   


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