Synchronous fluorescence spectra

Synchronous fluorescence spectra of all fractions were similar shown in Figure 3, but showed differences in relative intensity of four characteristic peaks at about 285,... 

The EEM fluorescence allows identifying major differences in fluorescent properties between fractions. However, the EEM fluorescence spectroscopy may become very time-consuming. For quick preliminary analysis, it is recommended that synchronous fluorescence spectra be used for distinguishing samples of DOM with different chemical properties. 

Cabaniss, S. E. 1992. Synchronous fluorescence spectra of metal-fulvic acid complexes. Environmental Science and Technology 26 1133. 

Fluorimetric measurements were carried out with a commercial Hitachi F-4500 spec-trofluorometer equipped with a 150 W xenon lamp and interfaced to a personal microcomputer for instrument operation and spectra processing. The slit-widths were adjusted at 2.5 nm both for excitation and emission and the photomultiplier (PMT R-3788) voltage set to operate at 950 V. The synchronous fluorescence spectra were corrected for variations with wavelength of the lamp intensity and photomultiplier sensitivity. 

Figure 4.3.6 Examples of front face synchronous fluorescence spectra obtained from tab-shaped (. s sorbent elements after immersion in Meuse River water spiked with a mixture of PAHs. The marked fluorescence bands are those that were used for measuring the concentrations of the individual PAHs...

FIGURE 15-7 Synchronous fluorescence spectra, in (a), the excitation and emission spectra of telracene are shown, in (b), the synchronous spectrum is shown for a fixed-waveiength difference of 3 nm, (From T. Vo-Dinh, Anal. Cherrh. 1978, 50, 396. Figure i, p. 397. Copyright 1978 American Chemical Society.)... 

Synchronous fluorescence spectra (wavelength difference - 34nm). Acid-hydrolyzed Isoamyl alcohol extracts of human placental DMA pooled from 8 donors. Emission wavelength at 344 corresponds with signature signal of BP tetrols. B) Eluant of Immunoaffinity column chromatography of acid-hydrolyzed pooled placental DMA. C) Eluant of Immunoaffinity column chromatography after additional clean-up with HPLC. D) Authentic sample of BP-7,10/8,9-tetrol. 

Li, FI., Zhang, Q., Zhu, Ch., Wang, X. (2008). Assessment of phytoplankton class abundance using in vivo synchronous fluorescence spectra. Anal. Biochem., 377, pp. 40-45, ISSN 0003-2697... 

Esteves Da Silva, J. C. G., and R. Tauler. 2006. Multivariate curve resolution of synchronous fluorescence spectra matrices of Mvic acids obtained as a function of pH. Applied Spectroscopy 60, no. 11 1315—1321. 

Figure 3.13. Synchronous fluorescence spectra of treated sewage effluent at 20 nm, 40 nm, and 60 nm offset values (AA). (Adapted from Galapate et al., 1998, with permission from Elsevier.)...

Cabaniss, S.E. and Shuman, M.S. (1987). Synchronous fluorescence spectra of natural waters Tracing sources of dissolved organic matter. Mar. Chemi., 21,37-50. 

Hur, J. and Kong, D.S. (2008). Use of synchronous fluorescence spectra to estimate biochemical oxygen demand (BOD) of urban rivers affected by sewage treatment. Environ. Technol., 29(4), 435-444. 

Figure 9.5. Synchronous fluorescence spectra with an offset of 65 nm of a freshwater sample (solid line) and marine water sample (dotted line). Locations of the a (humic-like), jS (marine humic-like), and 7 (tyrosine-like) peaks are identifled. The marine humic-like peak has been associated with recently created humic material due to algal activity and is commonly seen with a corresponding protein peak. The and a peaks are used to calculate the freshness index, which is considered an indicator of the degree of recently produced humic material in coastal systems. (From Parlanti et al. [2000], with minor adaptations.)...

Kalbitz, K., and Geyer, W. (2001). Humiflcation indices of water-soluble fulvic acids derived from synchronous fluorescence spectra - effects of spectrometer type and concentration, J. Plant Nutr. Soil Sci., 164(3), 259-265. 

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