Synchronized phagocytosis

Centrifuge plates at 4 °C at 500 x g for 8 min to synchronize phagocytosis. Spin all plates even if wells contain only S. aureus or only PMNs. 

This plating method conserves macrophages, ensures that each sample contains a similar number of cells and (most importantly) is compatible with synchronized phagocytosis. 

The following method of macrophage infection with either B. abortus or F. tularensis aims at synchronizing bacterial phagocytosis, which is important for subsequent intracellular trafficking analyses, as all bacteria have entered macrophages within a short time frame. 

Centrifugation of H. pylori onto macrophages at low temperature allows particle binding but not phagocytosis, and rapid transfer of samples to 37 °C supports synchronized ingestion. Aspirate medium from dishes of macrophages. Place 1 mL of fresh 37 °C medium into each dish and return dishes to the... 

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