Susceptibility of Orally Infected Japanese Mosquitoes

Fourteen mosquito species found commonly in Japan were studied on their susceptibility and transmission to the virus. Dengue type 2 virus (New Guinea C) was used for the susceptibility experiment. The stock virus was prepared as a 10% suspension of suckling mice brains (SMic) diluted with 2% fatal bovine serum (FBS). The stock viruses of mouse-passaged numbers 70 and 71 (mouse-adapted viruses) were used for the experiments. The virus titer was 10 -10 SMicLD5Q/ 

Mosquitoes were allowed to feed the virus solution (VBS), and suspensions of 10-pooled mosquitoes were inoculated intracerebrally into suckling mice immediately after the ingestion and after 20 days at 30 °C. 

Transmission of Mouse-Adapted or Non-mouse Passaged Dengue Viruses by the Japanese Mosquito Species 

Further Analysis for Vector Mosquitoes to Dengue Viruses in Japan 

3 Possible Vector of Japanese Mosquitoes Against West Nile Virus 1221 

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