Survey of the most common coupling procedures

Coupling of affinants on cyanogen bromide-activated support 

The most widely used activation procedure of agarose, dextran, but less common of cellulose and hydroxyalkyl methacrylate gel, employs cyanogen bromide [95]. The 

Total coupling capacity = 0.80 (cyanate esters) 0.15 (imidocarbonates) 

The attachment of amines on cyanogen bromide-activated Sepharose produces 

A -substituted isourea, which is capable of protonation in the neutral and alkaline region. In the presence of primary amines and ammonia this linkage is unstable [98]. This is the primary flaw of this method when it is used for a single attachment. 

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