Surface Biofunctionalization of Organosilica Nanoparticles

MPDMS nanoparticles are -50.2, -49.4 and —50.6mV, respectively. The zeta potentials of MPMS nanoparticles incorporating fluorescent dye (e.g. rhodamine red) within their interior are not markedly different from those nanoparticles 

Flow cytometry was performed to compare the surface properties of MPMS and TEOS nanoparticles in solution. When the nanoparticle solutions were mixed with protein solutions containing either green fluorescent protein (GFP) or phycoery- 

When the dispersion of MPMS nanoparticles modified with surface proteins was observed microscopically, the addition of GPP to a MPMS nanoparticle solution led to well-dispersed nanoparticles with a distinct fluorescence. This indicated that, compared to TEOS nanoparticles, the GPP modified the MPMS nanoparticles very effectively while retaining good dispersion. MPMS nanoparticles modified with GPP were also detected and observed using fluorescence microscopy. 

Figu re 4.8 Flow cytometry analysis of silica nanoparticles surface-modified with proteins. MPMS nanoparticles (a, c) and TEOS nanoparticles (b, d) modified with GFP (a, b) or phycoerythrin-conjugated streptavidin 

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