Suppliers Appendix

Use deionized or distilled water in all recipes and protocol steps. For common stock solutions, see APPENDIX 2A for suppliers, see SUPPLIERS APPENDIX. 

The following procedure is based on the Vectastain ABC kit from Vector (suppliers appendix). It uses an avidin-biotin complex to attach horseradish peroxidase (HRP) or alkaline phosphatase (AP) to the biotinylated secondary antibody. Avidin-biotin systems are capable of extremely high sensitivity because multiple reporter enzymes are bound to each secondary antibody. In addition, the detergent Tween 20 is a popular alternative to protein blocking agents when using nitrocellulose or PVDF membranes. 

Recipe is from Harlow and Lane (1988). Alternatively, BCIP/NBT substrates may be purchased from Sigma, Kirkegaard Perry, and Vector (SUPPLIERS APPENDIX). 

For common stock solutions, see APPENDIX 2A for suppliers, see SUPPLIERS APPENDIX. 

Ordering and product information can be found at the company websites (see SUPPLIERS APPENDIX). 

Special equipment is itemized in the materials list of each protocol. Listed below are standard pieces of equipment in the modem food science laboratory—i.e., items used extensively in this manual and thus not usually included in the individual materials lists. See suppliers appendix for contact information for commercial vendors of laboratory equipment. 

Appendix A Some Common Abbreviations Used in the Plastics Industry Appendix B Important Properties of Plastics and Listing of Plastics Suppliers Appendix C Important Properties of Elastomers... 

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