Superheated water liquid chromatograph

Miller and Hawthorne [416] have developed a chromatographic method that allows subcritical (hot/liquid) water to be used as a mobile phase for packed-column RPLC with solute detection by FID, UV or F also PHWE-LC-GC-FTD couplings are used. Before LC elution the extract is dried in a solid-phase trap to remove the water. In analogy to SFE-SFC, on-line coupled superheated water extraction-superheated water chromatography (SWE-SWC) has been proposed [417]. On-line sample extraction, clean-up and fractionation increases sensitivity, avoids contamination and minimises sources of error. 

R. M. Smith, R. J. Burgess, O. Chienthavorn, and J. R. Stuttard, Superheated water a new look at chromatographic eluents for reversed-phase liquid chromatography LC-GC Internal. 12 (1999), 30-36. 

S. F. Fields, C. Q. Ye, D. D. Zhang, B. R. Branch, X. J. Zhang, and N. Okafo, Superheated water as eluent in high-temperature high-performance liquid chromatographic separation of steroids on a polymer-coated zirconia column,/. Chromatogr. A 913 (2001), 197-204. 

Smith, R.M. Burgess, R.J. Chienthavom, O. Rose, J. Superheated water A new look at chromatographic eluents for reversed-phase liquid chromatography. LC-GC1999,17 (10), 938 945. 

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