Summary of Method Development Steps

Sequential isocratic Column Modern columns packed with C8 or 

Detection wavelength Use PDA to evaluate 1, of all analytes. Select kmax of main component or far-UV wavelength (200-230 nm). 

Mobile phase MPA Water for neutral analytes or acidified at pH 2-A for acidic or basic analytes MPB MeOH or ACN 

Conditions Evaluate first chromatogram to define gradient range A ) Set T = 30°C, F is defined by dc 

Isocratic or gradient Mobile phase and Evaluate method with impurity 

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