Sulphated polysaccharide extract

AhdEl-Baky, H. H., ElBaroty, S., and ElBaz, F. K. (2009). Potential biological properties of sulphated polysaccharides extracted from the macroalgae Ulva lactuca L. Acad. J. Cancer Res. 2,1-11. 

In a number of cases, e.g. for cloning and DNA sequencing, the DNA must be recovered from the gel in as high a yield as possible and without damage to the molecules. A number of methods have been tried, none entirely satisfactory, but electroelution is most often used. The two major problems with this method are, firstly, that the agarose is often contaminated by sulphated polysaccharides, which are extracted from the gel together with the DNA, and are potent inhibitors of many enzymes and, secondly, that the yield of DNA, particularly for large molecules, is relatively low. 

Wang, J., Zhang, Q., Zhang, Z., and Li, Z. (2008). Antioxidant activity of sulphated polysaccharide fractions extracted from Laminaria japonica. Int. ]. Biol. Macromol. 42,127-132. 

The identification of the mast cells recognizes local accumulations of highly concentrated heparin the chemical extraction requires large amounts of tissue. While a correlation between mast cell count and heparin content of tissues has not been established , the relationship between these two seems to be sufficiently close to indicate that all heparin is associated with mast cells. This does not prove that in ordinary tissue the mast cells contain the heparin and none is from the surrounding connective tissue matrix. Normal mouse and rat mast cells contain heparin and no other polysaccharides " , but solid mast cell tumours of mice also contain chondroitin sulphate-like polysaccharides, as an ascites subline of the Furth tumour and dog mastocytomas gave nonsulphated polysaccharide material. 

The complexes are not glycoproteins, but involve the adsorption of the enzyme on polysaccharide. The evidence for the association comes from the isolation from soil, using buffer extraction, of active enzyme fractions which, on hydrolysis, yield the usual rainge of neutral sugars common to soil. Dissociation of the enzymes (lysozyme and hyaluronidase) from the carbohydrate occurs on treatment with sodium dodecyl sulphate, which would not separate covalently linked components. This evidence does not rule out the possibility that the association occurs during coextraction. 

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