Sulfolobus factor

Durr H, Korner C, Muller M, Hickmann V, Hopfner KP (2005) X-ray structures of the Sulfolobus solfataricus SW12/SNF2 ATPase core and its complex with DNA. Cell 121 363-373 Eberharter A, Becker PB (2004) ATP-dependent nucleosome remodelling factors and functions. J Cell Sci 117 3707-3711... 

Aguilar, C.F., Sanderson, I., Moracci, M., Ciaramella, M., Nucci, R., Rossi, M., and Pearl, L.H. (1997) Crystal structure of the (1-glycosidase from the hyperthermophilic archeon Sulfolobus solfataricus resilience as a key factor in thermostability, J. Mol. Biol. 271, 789-802. 

The investigations outlined above demonstrate the utility of electrochemical techniques in probing cluster reactions. The titrimetric bulk electrolysis procedure allows new cluster types to be prepared easily, with quantitative information on stoichiometry. The cyclic voltammetry approach permitted a systematic study of other ferredoxins to determine the factors that allow rapid reactions of this type to occur. It could be shown, for example, that the presence of Asp in place of Cys in the sequence was not sufficient to confer this striking ambivalence between cluster types. The 7Fe ferredoxin from Sulfolobus acidocaldarius, which also has the... 

Cys-X-X-Asp-X-X-Cys sequence (Fig. 19) shows stable cyclic voltammetry in the presence of 0.1 raM Fe even at 60 °C (Armstrong FA, Butt JN, Cammack R, George SJ, Thomson AJ, unpublished results). Clearly in this case, the ability of [3Fe-4S]° to accept the fourth Fe is greatly diminished. Factors that may be influential include active-site exposure Sulfolobus Fd is much larger) and, perhaps, the presence, in D.a. Fdlll of a Glu instead of the usual Pro residue adjacent to the remote Cys coordinating the transformable cluster. Proline is well known as a creator of kinks or bends in the polypeptide chain. 

---