Sulfo-HSAB

Figure 5.20 Sulfo-HSAB is a short photoreactive crosslinker that can be used to modify amine-containing molecules through its NHS ester end to form amide linkages. After photoactivation, the phenyl azide group can react with amines to create a covalent bond.

HSAB and sulfo-HSAB have been used to investigate serum amyloid A (Cai et al., 2007), the functional role of C-terminal sequence elements in the transporter associated with antigen processing (Ehses et al., 2005), and the kinetics of intermolecular interactions during cytochrome C protein folding (Nishida et al., 2004). 

Although there are no reported applications for the cross-linker, its reactivity and use is similar to that of sulfo-HSAB. The commercial availability of the reagent provides additional options for spacer length to study the interactions between two proteins or other molecules. 

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