Sugars, in glycoproteins

Lehnhardt and Winzler [439] proceeded in a similar manner in determining neutral sugars in glycoproteins. Hydrolysis of 0.1—3 mg of the sample was performed on a... 

Wyss, D.F. and Wagner, G, 1996, The structural role of sugars in glycoproteins. Current Opinions in Biotechnology 7, 409-416. 

Waechter, C.J. and Lennarz, W.J. (1976). The Role of Polyprenol-Linked Sugars in Glycoprotein Synthesis . Annual Reviews of Biochemistry, 45, 95-112. 

Dansyl hydrazone (l.e. 5-dimethylamino-l-naphthalenesulphonic hydrazone) derivatives have been used for the reversed-phase analysis of neutral sugars in glycoprotein hydrolyzates, with the single peaks per sugar being detected at 254 nm, and of glucose... 

Polystyrenesulfonate cation-exchange resin, 55% DVB, H + -form Acetonitrile-water (23 2) separates neutral and amino sugars in glycoprotein hydrolysates None 30 Postcolumn reaction with 2- cyanoacetamide UV(280nm)... 

Waechter, C. j. and W. J. Lennarz, The role of polyprenol-linked sugars in glycoprotein synthesis, Annu. Rev. Biochem., 45, 95-112(1976). 

Threlfall, D. R. Polyprenols and terpenoid quinones and chromanols. In Encyclopedia of Plant Physiology, New Series, Vol. 8, Secondary Plant Products (E. A. Bell, B. V. Charlwood, eds.), pp. 288-308. Springer, Berlin-Heidelberg-New York 1980 Waechter, C. J., Lennarz, W. J. The role of polyprenol-linked sugars in glycoprotein synthesis. Annu. Rev. Biochem, 45. 95-112 (1976)... 

Gas Chromatography Analysis of Sugars in Glycoproteins of Blood Serum Chem. Zvesti 31(1) 87-91 (1977) CA 88 71075f... 

Determination of Neutral and Amino Sugars in Glycoproteins and Glyco-saminoglycans from Tissues and Biological Fluids... 

Mannose Hexose Man GDP-Man Common sugar in N-linked glycoproteins. 

N-Acetylneur-aminic acid Sialic acid (nine C atoms) NeuAc CMP-NeuAc Often the terminal sugar in both N- and 0-linked glycoproteins. Other types of sialic acid are also found, but NeuAc is the major species found in humans. Acetyl groups may also occur as 0-acetyl species as well as N-acetyl. 

V-Acetylneuraminic acid is a common group in glycoproteins, and it contains both the amide and carboxyl chromophores. As shown in formula 11, this nine-carbon sugar derivative has an equatorial amido group on C-5 and both a hydroxyl group and a carboxyl group on the anomeric carbon atom. 

The flexibility of the sugar linkages in glycoproteins results in multiple conformations that can be detected by time-resolved fluorescence experiments. Figure B9.1.1 shows an example of a triantennary glycopeptide labeled with a... 

Qy-boronate derivatives have the ability to form covalent bonds with d,v-diol sugars at alkaline pH. Unlike lectins, which typically bind only the terminal sugar in a complex carbohydrate, boronates can bind any physically accessible CM-diol on a glycoprotein.23-25 When hydrophobic boronate derivatives are used, the hydrophobicity of each complexation site is enhanced. To the extent that the protein components of a sample are differentially glycosylated, this can provide a tool for exploiting those differences. 

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