Sugars aminopyridine

A variety of reagents have been described for reductive amination, a labelhng reaction based on the attachment of an amino group at the carbonyl group in a reducing sugar under mild acidic conditions. This yields an acid-labile Schiff base (imine derivative), which is subsequently reduced to a stable secondary amine. Frequently applied reagents are 2-aminopyridine (PA), 2-aminoacridone (2-AMAC), and 4-aminobenzene derivatives such as 4-aminobenzoic ethyl ester (ABEE) or aminobenzoic butyl ester (ABBE). 

Later MD studies on DNA triplexes were often devoted to the stability and the H-bond pattern of the base triads, to the conformational properties of the sugar-phosphate backbone, to the effect of modified nucleotides and to the hydration pattern. Modified nucleotides include structures derived from 2-aminopyridine and 8-aminoguanine a triplex with the phosphodiester linkage in two strands replaced by S-methylthiourea linkers, 2 -aminoethoxy-substituted riboses and a -CH2-lengthening of the intemucleotide linkage. ... 

Aminopyridine reacts with reducing sugars to form Schiff bases that can be reduced to fluorescent 2-amino-pyridyl derivatives [408], This reaction has been used for the determination of pmol quantities of neutral and amino sugars and of muraminic acid [409]. 

As alditol pernaphthoates Reductive amination With ABEE With 2-aminopyridine With anthranilic acid With ANTS or AMAC Reducing sugars Reducing sugars 0.1 pmol 0.5 nmol 10 pmol lOOfmol 0.2 pmol ... 

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