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Sugar phosphates pentose phosphate preparation

Figure 3 Conversion of ribose S-phosphate to sugar phosphate products by rat liver enzyme preparation (RLEP) (40). Details of the incubation, protein content and methods used are described in (42). For measurement of the max. catalytic capacity of RLEP see (43). Reprinted from International Journal of Biochemistry, 19. Williams John. F., Arora Krishan K. and Longnecker John P. The pentose pathway A random harvest, 69 Pages, 1987, with permission from Elsevier, http //www.sciencedirectcom/science/journal/l 3S7272S... Figure 3 Conversion of ribose S-phosphate to sugar phosphate products by rat liver enzyme preparation (RLEP) (40). Details of the incubation, protein content and methods used are described in (42). For measurement of the max. catalytic capacity of RLEP see (43). Reprinted from International Journal of Biochemistry, 19. Williams John. F., Arora Krishan K. and Longnecker John P. The pentose pathway A random harvest, 69 Pages, 1987, with permission from Elsevier, http //www.sciencedirectcom/science/journal/l 3S7272S...
Transketolase (EC 2.2.1.1) is involved in the oxidative pentose phosphate pathtvay in tvhich it catalyzes the reversible transfer of a hydroxyacetyl nucleophile bettveen a variety of sugar phosphates. The enzyme, tvhich requires thiamine diphosphate and divalent Mg as cofactors [248], is commercially available from baker s yeast and can be readily isolated from many natural or recombinant sources [249, 250]. The yeast enzyme has been structurally tvell characterized [251], including protein tvith a carbanion intermediate covalently bound to the cofactor [252]. Large-scale enzyme production has been investigated for the transketolase from Escherichia coli [253-255]. Immobilization vas sho vn to significantly increase stability against inactivation by aldehyde substrates [256]. The enzyme is quite tolerant to organic cosolvent, and preparative reactions have been performed continuously in a membrane reactor [255], vith potential in-situ product removal via borate complexation [257]. [Pg.247]

A mixture of pentose phosphates has been found among the reaction products of phosphogluconate degradation. The first analysis of these products by Scott and Cohen following the use of the enzyme preparation of Dickens revealed a small proportion of ribose-5-phosphate, the pentose of which was identified by paper chromatography and adaptive enzymic analysis. However, most of the pentose phosphate which was isolated on paper and subsequently as the free sugar was a previously uncharacterized substance. Horecker and Smyrniotis used a considerably purified enzyme preparation, and pentose phosphate was produced under conditions in which the following stoichiometry prevailed ... [Pg.200]


See other pages where Sugar phosphates pentose phosphate preparation is mentioned: [Pg.217]    [Pg.47]    [Pg.146]    [Pg.142]    [Pg.224]    [Pg.1418]    [Pg.204]    [Pg.290]    [Pg.54]    [Pg.41]    [Pg.936]    [Pg.11]    [Pg.453]    [Pg.16]    [Pg.28]   
See also in sourсe #XX -- [ Pg.210 ]

See also in sourсe #XX -- [ Pg.49 , Pg.210 ]




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Sugar phosphates

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