Substrate Scope, Activity, and Enantioselectivity

HNLs often exhibit only low activity toward industrially relevant compounds. Thus, a lot of effort was (and still is) made to engineer HNLs for the acceptance of unfavorable substrates by rational design and directed evolution. 

TABLE 25.2 Summary of Engineered HNL Variants Exhibiting Improved Properties  

PaHNL5 LlQ/AlllG Improved yield and ee in the synthesis of (R)-2-chloromandelonitrile [37] 

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