Subretinal fluid reabsorption

The effects of INS37217 on subretinal fluid reabsorption were evaluated in mice, rats, and rabbits by injecting saline solution into the subretinal space using a small needle (32 gauge or less), which produces a non-RRD because the induced retino-tomy appears to seal itself immediately (3). This model of induced retinal detachment was chosen because RRDs occur too infrequently in animals to be useful for precli-nical proof-of-concept studies. 

The effects of INS37217 on subretinal fluid reabsorption in rabbits were made in a similar manner (4). As with the rat studies, a single non-RRD was produced in New Zealand white rabbits by injecting modified PBS ( 50pL administration volume) solution in the subretinal space, which resulted in a detachment that was less than 10% of the retinal surface area of the rabbit eye. Immediately following the creation of the subretinal bleb, an intravitreous injection (50 pL) of saline alone or... 

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