Subject microtiter plate-based

A number of assays for the hydrolase-type of Hyals were developed in the last decade that facilitated their characterization. These include microtiter-based ELISA-like assays, in which a highly specific HA-binding protein substitutes for the antibody component [186]. Biotinylated HA bound to microtiter plates is subjected to Hyal activity, and the remaining HA quantified by an avidin-enzyme color reaction [107]. HA substrate gel zymography procedures were also formulated that facilitated additional studies of these enzymes [187]. 

A systematic approach to profiling active metabolites using a 96-well plate format was recently described (Shu et al., 2002). The approach is based on a rapid bioassay-guided metabolite detection and characterization. Drug metabolite mixtures (generated by various methods described below) are separated and fractions collected into microtiter plates such as 96-well plates. The fractions are then subjected to one or more relevant activity (e.g., receptor ligand binding) assays. 

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