Subfractions quantitative precipitin

Figure 3. Quantitative precipitin reactions between the mannan subfractions (Batch I) and antisera of three C. albicans strains and S. cerevisiae (A) anti-C. albicans NIH A-207 (A-strain) serum (B) anti-C. albicans NIH B-792 (B-strain) serum (C) anti-C. albicans J-1012 (J-strain) serum (D) anti-S. cerevisiae serum. Mannan subfractions are of (O) A-strain (0) B-strain (A) J-strain (A)...

Figure 6. Quantitative precipitin curves of five mannan subfractions against homologous anti-whole-cell serum of parent bakers yeast (O) Fraction A (%) Fraction B (l ) Fraction C ( ) Fraction D ( j Fraction E O.D., optical density (5).

The sensitivity and specificity of the quantitative precipitin method make it applicable to the identification and estimation of abnormal plasma proteins in certain diseases, particularly when present in concentrations too low to be detected by other methods. Goettsch and her associates (103, 104) and Kendall (181) employed immunochemical techniques in the study of nephrotic sera Kendall (181) also investigated the distribution of his immunologically distinct globulin subfractions in hyperglobulinemia due to lymphogranuloma venereum, cirrhosis of the liver, and rheumatoid arthritis Kabat (261) was able to demonstrate the presence of as little as 0.15 gram per cent Bence-Jones protein in the serum of a patient with multiple myeloma. Many other applications of immunochemical techniques are noted by Kabat (167) and Treffers (358) in recent reviews. 

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