Subcellular analysis organelle preparation

In spite of the reduction in complexity that can be achieved by cellular fractionation, an analytical separation is frequently required to separate one or more components from the cellular milieu. As evidenced throughout this book, capillary electrophoresis (CE) provides high resolution and separation efficiency, both of which are necessary for subcellular analysis. In addition, CE is advantageous because it requires very little sample volume, typically less than a nanoliter, and generally very little sample preparation. Hence, capillaries have been used to directly sample subcellular compartments within neurons, oocytes, and muscle tissue sections. They have also been used to analyze individual organelles from a single cell following on-column lysis." ... 

Many complex cellular processes are best smdied through the use of an appropriate cell model. Cell cultures provide a relatively stable and virtually unlimited supply of organelles for subcellular analysis. We will discuss organelle preparations that are commonly used in the author s laboratory... 

Subcellular fractionation, sucrose density gradients Allows isolation of specific cellular organelles for organelle proteome analysis difficult to obtain completely pure preparations... 

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