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Subbed slides

Fix the cell bearing coverslips to slides as above. The use of subbed slides is unnecessary. [Pg.253]

Transfer ribbons of sections into 0.2 X subbing solution on subbed slides. Place on a slide warmer at = 45 C to stretch the sections, remove excess subbing solution and dry at 42°C for 24 h. [Pg.253]

After 15 min, trim the block, section the block and collect sections onto warm subbed slides or coverslips coated with Histostik (Accurate Chemical and Scientific, Co.) with standard methods. [Pg.253]

Cut sections (5-20 (tm) of unfixed tissue in a cabinet cryostat (-20 C ) directly onto subbed slides. Allow the sections to air dry overnight at room temperature in a dark, dust-free place. Alternatively, sections on slides may be stored at -TO C to -80°C (2-3 mo) in a sealed plastic slide box. Stored slides should be allowed to warm to room temperature before use. [Pg.51]

Tissue sections are obtained from rats that are killed by decapitation. The brains (or other required tissue) are rapidly removed and frozen in isopentane that has been precooled (-30°C) on dry ice. Sections (14 pM) are cryostat-cut and mounted (two sections per slide) under conditions that minimize RNase contamination. Mounting sections close to the bottom of the subbed slides will conserve reagents required for double-label ISH. Multiple adjacent series of sections are obtained, depending on need. For example, it is advisable to take a series for each [ Sj-labeled riboprobe both alone and in combination with each appropriate digoxigenin-labeled riboprobe. Slide-mounted sections are sorted at -20°C (in cryostat or cold room) prior to the assay to obtain the required number of near adjacent series. Slides brought to room temperature cannot be... [Pg.81]

Stir each emulsion with clean glass rods and dip a clean subbed slide up and down ( 50 times) to remove air bubbles. Check for a homogeneous coating of emulsions by dipping clean subbed slide in each respective emulsion. [Pg.85]

It is not necessary to use subbed slides in this procedure because the roots have not been treated with enzymes that may prevent adherence of the cells to the slide. [Pg.152]

OCT (TissueTek) and gelatin-subbed slides or Superfrost Plus slides (Fisher). [Pg.327]

SUBBED SLIDE WITH RECEPTOR LABELED TISSUE... [Pg.303]

Subbed slides dip racks of microscope slides into a mixture of 1% gelatin plus 1% formalin in distilled water drain the slides and dry in a warm oven... [Pg.252]

Thaw the drop and touch onto a subbed slide. [Pg.253]

Sections are floated on a clean water bath filled with distilled water at a temperature slightly below the melting point of the wax. After the sections spread out, subbed slides are placed beneath the floating ribbons to collect them and dried on a 37°C heating plate for 1 h, and then overnight in a 37°C oven. [Pg.255]

Wash slides, dip in subbing solution, and allow to dry in a dust-free place. These slides may be stored indefinitely in a dry, dust-free container. Subbed slides give a better retention of cytological preparations. [Pg.123]

Invert a subbed slide over the coverslip and press it down lightly. [Pg.124]

Cut sections (10-14-pm thick) and transfer to slides (subbed slides are not necessary). ... [Pg.235]

Transfer sections to a subbed slide (see Protocol 13.4 Notes, below) by rolling sections first onto a beveled wooden stick and then off of the stick into a puddle of H O on the subbed slide. [Pg.239]

Subbed slides are not absolutely necessary, but because the subbed surface increases the surface tension of H O, the drop of H O forms a bead rather than spreading out in a thin film across the slide. This is convenient because the sections are consequently confined to a smaller surface area on the slide. [Pg.240]


See other pages where Subbed slides is mentioned: [Pg.87]    [Pg.117]    [Pg.260]    [Pg.249]    [Pg.249]    [Pg.250]    [Pg.254]    [Pg.255]    [Pg.257]    [Pg.260]    [Pg.123]    [Pg.231]    [Pg.233]    [Pg.234]    [Pg.237]    [Pg.324]   
See also in sourсe #XX -- [ Pg.252 ]




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