Studies Conducted in Vivo with Isotopes

An accoimt of the early work of Zabin and Mead (1953, 1954) and Sprinson and CouLON (1954) on the incorporation of carbon-14 labeled acetate and serine 

While the first mention of a sulfolipid in brain tissue was in 1884 (Thudichum), definitive studies on the structure of cerebroside sulfates are still in progress (Yamakawa et al. 1962 Stoffyn and Stoffyn 1963 Taketomi and Yamakawa 1964). Similarly, the metabolism of cerebroside sulfates is largely unknown. Brain 

Other authors have confirmed the radioactive sulfate incorporation data (Green and Robinson, 1960 Bakke and CoRNATZER, 1961 Davison and Gregson, 1962 Kataoka, 1962 and Burton, unpublished data). While Burton (unpublished data) has obtained results consistent with those of Rad in s for the persistence of radioactive sulfate in the cerebroside sulfate fraction. Burton s experiments indicate the same rate of loss of radioactivity due to in the carbohydrate moieties of cerebroside and cerebroside sulfate whereas Radin et al. found a retension of as well as Hauser (1964) and others (Bakke and Cornatzer, 1961, Davison and Gregson, 1962) have observed that the cerebroside cerebroside sulfate ratio in brain tissue appears to remain constant even though these lipids accumulate with age. Thus Hauser (1964) found ratios of 3 in 20 day-old rats and 4 in adult animals. Further experiments need to be carried out to determine 

1963) has yielded much interesting data. Ha JBA and Rad in (1963 a, b) injected weanling rats with tritium-labeled acetate, the animals were killed and the cerebrosides were isolated from the brain tissue.The fatty acids of the cerebrosides 

Thereafter, a slow increase in total radioactivity of the 20 0, 22 0, and especially the 24 0 acids occurred. 

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