Structural—functional investigations based measurements

Structural-Functional Investigations Based on Partial Proteolysis and Physical Measurements... 

At Johnson Johnson, the structure of oxidized cellulose was investigated by several techniques. Chemically, a differential titration was developed to measure the carboxylic acid content and any saponifiable secondary functions. The titration measures carboxy lic protons by ion exchange. By treating the resulting ion exchanged solution with a measured excess of base, the saponifiable function is measured by acidimetric back-titration. Using the differential titration, a previously measured 20 wt% carboxylic acid content is revealed to be 18 wt% carboxylic acid and 2 w t% saponifiable moiety (unpublished). 

Basic concepts of interaction have been refined as new models could be established. The fields of applications were broadened. Currently, the detection of enantiomers by fluorescence measurements and by direct optical methods are well-investigated topics. By introducing various functional groups to established skeletal structures, highly specific recognition of enantiomers is likely to be achieved. Those methods based on recognition by polymer-bonded selectors will continue to profit from advances in modern chromatographic methods. 

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