Structural comparison of rubredoxin and desulforedoxin

YEYDPAKGDPDSGI KPGTKFEDLPDDWA Yl YNPEDGDPDNGVNPGTDFKDI PDDWV QVVK.VLEEGGGTL V 

APKSE FEPA - -APKSEFEAA--VSKDQFSPA - -ASKDAFEKQ--VGKDQFEEVEE - - E D M V K Q - - - 

4 Single-metal replacement data in rubredoxin and desulforedoxin and crystal structures 

The conserved iron binding motif, Cys-X-X-Cys, found in Rd and in Dx and known as the rubredoxin knuckle , is an excellent structural model for the metal environment of members of the zinc finger family [42,43]. An attractive property of 

Spectroscopic studies were performed on Ni- and Co-substituted Rds and Dx [50] while Fe replacement was done for Mossbauer analysis [35]. The Dx derivatives containing Zn and Cd in the protein s metal site have been analy2ed by 2D-NMR spectroscopy and the solution structures have been determined [38, 39]. NMR studies were also done with the Zn, Cd and Hg derivatives for Rd [9, 22, 51, 52]. The Ni-containing derivative is of particular interest, as Ni-substituted Rd and Dx were shown to mimic the reactivity pattern of Ni-containing hydro-genases with respect to hydrogen production, deuterium-proton and inhibition by carbon monoxide [53]. However, there is no three-dimensional structure yet available for either the Rd or the Dx Ni-substituted proteins. 

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