Structural analysis of monomeric cyclic fatty acids

1 Structural analysis of monomeric cyclic fatty acids 

Until recently, a complete analysis of CFAMs from heated oils was a daunting task first, because of the complexities of the mixtures and, second, because of the difficulties in obtaining full structural information, especially with respect to double bond positions. However, recently this has been made possible by combining fractionation of a CFAM mixture (obtained by urea adduction) by silver-ion HPLC with GC-MS analysis of the picolinyl esters or DMOX derivatives of the fractions (Fig. 5.10 Christie et al, 1993  

Total CFAM phenacyl esters Silver-Ion HPLC CFAM phenacyl ester fractions 

CFAM methyl esters GC-FTIR (double-bond geometry) 

Hydrogenated CFAM methyl esters GC (geometry of side-chains about ring) 

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