Streptomyces pristinaespiralis

Sezonov, G., V. Blanc, N. Bamas-Jacques, A. Friedmann. J.L. Pernodet and M.Guerineau (1997). Complete conversion of antibiotic precursor to pristinamycin IIA by overexpression of Streptomyces pristinaespiralis biosynthetic genes. Nature Biotechnol. 15 349-353. 

POULENC using a selected strain of Streptomyces pristinaespiralis (see Scheme... 

The biosynthesis of pristinamycin 1 by Streptomyces pristinaespiralis is currently being studied in the RHONE POULENC laboratories. The natural amino acids threonine and proline have been shown to be incorporated directly into pristinamycin 1 and radiolabeled " C-pristinamycin I a has been prepared by incorporation of [U- C]proline. Interesting studies on the biosynthesis of virginiamycin Si described by Kingston and co-workers [44] showed that the phenylglycine residue was derived from phenylalanine via an intermolecular transfer of nitrogen. More recently, they have demonstrated that both the (S)-4-oxopipecolic acid (8) and 3-hydroxypicolinic acid (9) residues are derived from lysine via the two possible pathways depicted in Scheme 2 in which the... 

The biosynthesis of pristinamycin 11 by Streptomyces pristinaespiralis is currently being studied in the RHONE-POULENC laboratories. Previous studies [90, 91] on the biosynthesis of virginiamycin Mi( = pristinamycin Ilyv, see Table 7) by Streptomyces virginiae identified acetic acid, valine, glycine, proline and serine as the natural building blocks of virginiamycin Mj (see Fig. 10). The 32-methyl was shown to be derived from methionine and the 33-methyl from the methyl of a molecule of acetic acid. 

Cephalosporium acremo nium dn Streptomyces pristinaespiralis dn Bacillus subtilis dn... 

Pristinamycin is a member of the streptogramin class of antibiotics effective against vancomycin-resistant S. aureus and vancomycin-resistant enterococcus. It is biosynthesized by the bacterium Streptomyces pristinaespiralis and consists of two components, pristinamycin lA (a macro-lide) and the depsipeptide pristinamycin HA (also known as streptogramin A) in the ratio of 30 70. Streptogramins, also called synergistins, are made up of two groups, streptogramins A and B. 

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