Streptomyces badius

Production and Characterization of Water-Soluble Intermediates Derived from Streptomyces badius and Streptomyces viridosporus... 

Quinoxaline is a by-product of cooking some food and can inhibit the growth of some ciliate protozoa and plan-pathogenic fungi. The metabolism of this ring system by Pseudomonas putida can provide the quinoxaline cis-5,6-dihydrodiol, 5-hydroxyquinoxaline, and 2(l//)-quinoxalinone. When quinoxaline is metabolized by Streptomyces badius, two different products were isolated. Both of these two products, 2(l//)-quinoxalinone and 3,4-dihydro-2(l/ )-quinoxalinone, were only isolated from the treatment of Streptomyces badius with quinoxaline. Six other Streptomyces species were tested and only produced 2(l//)-quinoxalinone. The mechanism for the formation of 3,4-dihydro-2(l//)-[Pg.548]

Two Streptomyces strains, S. badius and S. viridosporus, were found to be able to grow on kraft lignin (In-dulin ATR) as sole carbon source. The resulting APPL (Acid Precipitable Polymeric Lignin) was characterized by FTIR and elemental analysis for C, H and N, and was found to contain proteins in addition to a relatively demethoxylated lignin component. The proteins were further characterized by amino acid analysis, while the lignin component was separated by solvent extraction and its molecular weight distribution determined by HPSEC. 

Crawford and co-workers were the first to study in detail the action of two streptomyces, S. badius 252 and S. viridosporus T7A, on lignocellulose from different sources. Their work led to the conclusion that the bacterial action on aqueous suspensions of these lignocellulosics resulted in the solubilization of lignin fragments which precipitate upon acidification Acid Precipitable Polymeric Lignin (APPL) (1-10). 

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