Streptolysin Activation

Storage of purified streptolysin O. The eluate from the hydrophobic chromatography column may be aliquoted and kept frozen at-70°C. Freeze drying of purified streptolysin O, without loss of activity (even of dilute samples), is possible if 0.1 % (w/v) bovine serum albumin is added. 

Weller, U, Muller L, Messner M et al. (1996) Expression of active streptolysin-O in E. coli as MBP-SLO fusion protein. The N-terminal 70 amino acids are not required for hemolytic activity. In EurJ Biochem, 236 34—39. 

Pinkney M, Beachey E, Kehoe M (1989) The thiol-activated toxin streptolysin O does not require a thiol group for activity. Infect. Immun. 57 2553-2558. 

Walev I, Hombach M, Bobkiewicz W, Fenske D, Bhakdi S, Husmann M (2002) Resealing of large transmembrane pores produced by streptolysin O in nucleated cells is accompanied by NF-kappa B activation and downstream events. FASEB J 16(2) 237-239... 

Sawada, S., Shida, M., Suenaga, R., Mizuma, H., Karasaki, M., Hashimoto, M., Kawano, K., and Amaki, I. (1986) Elicited antibody nature of human monoclonal protein with anti-streptolysin O activity-analysis with monoclonal anti-idiotype antibody. J. Clin. Lab. Immunol. 19, 31-35. 

In contrast to ascorbic acid and a-tocopherol amidothionophosphates did not have any prooxidative effects as measured by oxygen consumption from buffer solutions containing the drug and cupric sulphate as a source of redox-active metal ions (Ti-ROSH et al. 1996). Amidothionophosphates reduced significantly and in a dose-dependent manner the oxygen burst in human neutrophils as measured by luminol-dependent luminescence, and they also markedly depressed the killing of human fibroblasts by mixtures of glucose oxidase and streptolysin S. The toxicity of these molecules was tested by intraperitoneal injection of doses up to 1000 mg/kg to white Sabra mice. No mortality was observed 30 d after administration of up to 500 mg/kg. 

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