Streptavidin/horseradish peroxidase,

Streptavidin-horseradish peroxidase (HP) conjugate (Dako) Store at +4°C. Working solution dilute 1 600 in IX TBS prior to use. 

Fig. 27 a Poly(DiynePC) vesicles doped with DiynePE-biotin and Diyne-InsP . b Schematic of an assay using these vesicles. Proteins containing PIPn binding domains are spotted on a nitrocellulose membrane, then the membrane is incubated with polymerized vesicles, followed by streptavidin-horseradish peroxidase (SAHRP) and chemiluminescent detection. Reprinted with permission from [120], Copyright 2005, American Chemical Society... 

Sadumi, R, Alagon, A., Ahev, R. et al. 2005. Immobihzation of streptavidin-horseradish peroxidase onto a biotinylated polyfacryhc acid) backbone that had been radiation—Grafted to a PTFE film. J Biomat Sci Polym E. 16 181-187. 

With the ELISA-Hke competition assay, it is possible to determine and compare the competitive potential of different chemokines for the same GAG ligand. Instead of a capturing antibody, as it is used in a classical ELISA, GAGs are coated on specially prepared GAG-binding plates (Iduron) that adsorb GAGs without further chemical modifications while retaining their natural protein-binding characteristics. Biotinylated chemokines are incubated on these plates and can be fiirther competed off with other nonlabeled chemokines, proteins, or mutants. Therefore, biotinylation of the chemokines is an essential first step. After competition, the nondisplaced biotinylated chemokine is detected with streptavidin horseradish peroxidase... 

Streptavidin-horseradish peroxidase conjugate reagent (Amersham). 

Incubate membrane with streptavidin-horseradish peroxidase (1 5,000 in PBST) for 1 h at RT. 

Esseghaier, C., Bergaoui, Y, ben Fredj, H., Tlili, A., Helali, S., Ameur, S., and Abdel-ghani, A. (2008) Impedance spectroscopy on immobihzed streptavidin horseradish peroxidase layer for biosensing. Sens. Actuators B, 134,112-116. 

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