Protein binding characteristics

Bolte G, Osman A, Mothes T, Stern M. Peptic-tryptic digests of gliadin Contaminating trypsin but not pepsin interferes with gastrointestinal protein binding characteristics. Clin Chim Acta 1996 247 59-70. 

Barre J, Chamourad JM, Houin G, Tillement JP (1985) Equilibrium Dialysis, Ultrafiltration and Ultracentrifugation Compared for Determining the Plasma-Protein Binding Characteristics of Valproic Acid. Clinical Chemistry... 

Recent research on the speciation and protein binding characteristics of aluminum has led to a better insight in the mechanisms underlying the element s tissue distribution and toxicity [33,34]. In this context, the provocative hypothesis linking the widespread use of erythropoietin to the increasing prevalence of adynamic bone disease is of particular interest [35]. 

Nitrocellulose DNA and RNA binding Good for biological applications with higher protein-binding characteristics. + ++... 

Kodama, H. et al., Effect of temperature on serum protein binding characteristics of phenytoin in monotherapy paediatric patients with epilepsy, J. Clin. Pharm. Thera., 26, 175, 2001. 

It, therefore, seems clear that the molecular modification of progesterone, altering its solubility, distribution ratio, polarity, protein- binding characteristics, steric requirements, crystalline structure, and rate of metabolism (as well as the nature of the metabolic products themselves), would alter some of the properties of progesterone, eliminate some, and introduce new and unexpected properties, many of which may not be evident even after extensive animal studies. 

Melarsoprol is administered IV in multiple doses and multiple sessions. Its major metabolite in humans is the lipophilic melarsen oxide, which can penetrate into the CNS. This metabolite apparently is responsible for the protein-binding characteristic for melarsoprol. 

Lactation — Based on the high-protein-binding characteristic of TGB, infants may not have measurable plasma concentrations when the mother is receiving doses of TGB. However, there are no data available. 

Lactation — Based on the protein-binding characteristic of TOP, infants would be expected to have clinically significant plasma concentrations if the mother is taking therapeutic doses of TOP. The risk/benefit of breastfeeding needs to be discussed with the mother. 

With the ELISA-Hke competition assay, it is possible to determine and compare the competitive potential of different chemokines for the same GAG ligand. Instead of a capturing antibody, as it is used in a classical ELISA, GAGs are coated on specially prepared GAG-binding plates (Iduron) that adsorb GAGs without further chemical modifications while retaining their natural protein-binding characteristics. Biotinylated chemokines are incubated on these plates and can be fiirther competed off with other nonlabeled chemokines, proteins, or mutants. Therefore, biotinylation of the chemokines is an essential first step. After competition, the nondisplaced biotinylated chemokine is detected with streptavidin horseradish peroxidase... 

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