Streptavidin-coated polyurethane-urea nanoparticle

Multifunctional biotinylated and streptavidin-coated polyurethane-urea nanoparticles have also been engineered from OAV nano-emulsions." " In these studies, biotin or streptavidin reacts with diisocyanate at the droplet interface and are successfully attached to the nanoparticle polymeric matrices. These nanoparticles exhibit diameters around 110-140mn (biotin nanoparticles) and 70-74nm (streptavidin nanoparticles). In addition, streptavidin-coated polyurethane-urea nanoparticles were functionalized with biotin anti-VCAM-1 and anti-ICAM-1 antibodies for specific targeting. Both nanoparticulate systems showed no cytotoxicity in healthy endothelial cells and therefore good biocompatibility properties. Figure 7.1 shows a schema of the synthesis of different polyurethane nanoparticles obtained from 0/W nano-emulsions by interfacial polymerization and images obtained by transmission electron microscopy. 

Figure 7.1 Fonnation of different polyurethane nanoparticles (naked polyurethane, pegylated pol5mrethane, lysine-coated polyurea, biotinylated polyurethane-urea/pc DNA complex, and functionalized streptavidin-coated polyurethane-urea nanoparticles) obtained by interfacial polycondensation from O/W nano-emulsions (a) and transmission electron micrographs of polyurethane nanoparticles after being negatively stained with 1% v/v uranyl acetate (b).

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