Strategy for an Industrial Preparative Chromatography Laboratory

The effort required to develop a separation method strongly depends on several factors  

1) Recurrence of a separation problem (repeatedly or only once) 

3) Do we have to isolate a single target component or all relevant peaks in the mixture  

Obviously, when separating small quantities of many different products, standard separation methods using gradient elution are preferred. Conversely, for larger scale separations it is important to design a robust separation process that can run fully automated 24 h a day. Clearly, for a production-scale process that has to be performed under cCMP conditions. Many experiments are required to be able to design a reproducible, robust process that can be transferred from one production location to another without the need for substantial modifications. For this reason two examples, covering both separations are exemplified. 

Example 3.1 Separation of Large Numbers of Different Products in the Range 1-lOg 

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Strategy for an Industrial Preparative Chromatography Laboratory 145 4.3.3 Criteria for Choosing RP Systems 150... 

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